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| Registros recuperados : 22 | |
| 5. |  | MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; BASSO, M. F.; HENNING, L. M. M.; FUGANTI-PAGLIARINI, R.; HARMON, F. G.; NEPOMUCENO, A. L. Functional characterization of a putative Glycine max ELF4 in transgenic Arabidopsis and its role during flowering control. Frontiers in Plant Science, v. 8, artigo 618, 2017.| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; BASSO, M. F.; MERTZ-HENNING, L. M.; FUGANTI-PAGLIARINI, R.; HARMON, F. G.; NEPOMUCENO, A. L. Functional characterization of a putative Glycine max ELF4 in transgenic arabidopsis and its role during flowering control. Frontiers in Plant Science, v. 8, artigo 618, 2017.| Biblioteca(s): Embrapa Soja. |
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| 7. | | REIS, R. R.; NAKAYAMA, T.; MARIN, S. R. R.; MARINHO, J. P.; MARCOLINO-GOMES, J.; MERTZ-HENNING, L. M.; MOLINARI, H. B. C.; NEPOMUCENO, A. L. Engenharia genética de precisão para tolerância a seca em soja. In: CONGRESSO BRASILEIRO DE SOJA, 7.; MERCOSOJA, 2015, Florianópolis. Tecnologia e mercado global: perspectivas para soja: anais. Londrina: Embrapa Soja, 2015. 3 p. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 8. | | GOMES, J. M.; NAKAYAMA, T. J.; REIS, R. R.; RODRIGUES, F. A.; MERTZ-HENNING, L. M.; FUGANTI-PAGLIARINI, R.; MOLINARI, H. B. C.; HARMON, F.; NEPOMUCENO, A. L. Gene expression and functional analysis of soybean genes with diurnal oscillation during drought stress. In: PLANT & ANIMAL GENOME XXIII, 2015, San Diego. PO 203| Biblioteca(s): Embrapa Soja. |
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| 9. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; HARMONAND, F. G.; NEPOMUCENO, A. L. Gene expression networks and cis-elements combinatorial models in soybean: circadian clock and drought responses. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 45-46.| Biblioteca(s): Embrapa Soja. |
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| 10. | | RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; LOBO, F. P.; HARMON, F. G.; NEPAMUCENO, A. L. Daytime soybean transcriptome fluctuations during water deficit stress. BMC Genomics, v. 16, n. 505, p. 2-19, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| 11. | | RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; LOBO, F. P.; HARMON, F. G.; NEPOMUCENO, A. L. Daytime soybean transcriptome fluctuations during water deficit stress. BMC Genomics, v. 16, n. 505, 2015. 19 p.| Biblioteca(s): Embrapa Soja. |
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| 12. | | NAKAYAMA, T. J.; RODRIGUES, F. A.; MARCOLINO, J.; MARCELINO, F. C.; SILVA, F. R. da; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L.; NEUMAIER, N. Transcriptome analysis of soybean (Glycine max L. Merrill) root submited to hypoxic condition. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 154-155.| Biblioteca(s): Embrapa Soja. |
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| 13. | | MARTINS, P. K.; NAKAYAMA, T. J.; RIBEIRO, A. P.; DIAS, B. B. A.; NEPOMUCENO, A. L.; HARMON, F. G.; KOBAYASHI, A. K.; MOLINARI, H. B. C. Setaria viridis floral-dip: a simple and rapid Agrobacterium-mediated transformation method. Biotechnology Reports, v. 6, p. 61-63, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| 14. | | GOMES, J. M.; RODRIGUES, F. A.; PAGLIARINI, R. F.; BENDIX, C.; NAKAYAMA, T. J.; CELAYA, B.; MOLINARI, H. B. C.; OLIVEIRA, M. C. N. de; HARMON, F. G.; NEPOMUCENO, A. Diurnal oscillations of soybean circadian clock and drought responsive genes. Plos One, v. 9, n. 1, p. 1-13, 2014.| Biblioteca(s): Embrapa Agroenergia. |
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| 15. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI; BENDIX, C.; NAKAYAMA, T. J.; CELAYA, B.; MOLINARI, H. B. C.; OLIVEIRA, M. C. N. de; HARMON, F. G.; NEPOMUCENO, A. L. Diurnal oscillations of soybean circadian clock and drought responsive genes. Plos One, v. 9, n. 1, e86402, Jan. 2014. 13 p.| Biblioteca(s): Embrapa Soja. |
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| 16. | | MARCOLINO, J.; RODRIGUES, F. A.; AMARAL, L. C.; NAKAYAMA, T. J.; PEREIRA, R. M.; NASCIMENTO, L. C. do; BINNECK, E.; OLIVEIRA, M. C. N. de; MARCELINO, F. C.; ABDELNOOR, R. V.; NEUMAIER, N.; FARIAS, J. R. B.; NEPOMUCENO, A. L. Transcription factors expressed in soybean roots in response to water déficit. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 193-194.| Biblioteca(s): Embrapa Soja. |
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| 17. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. Plos One, v. 10, n. 9, e0139051, 2015. 16 p.| Biblioteca(s): Embrapa Agroenergia; Embrapa Soja. |
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| 18. | | NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L. Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 1, p. 860-871, 2014.| Biblioteca(s): Embrapa Clima Temperado; Embrapa Soja. |
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| 19. | | RODRIGUES, F. A.; MARCOLINO, J.; ENGELS, C.; NAKAYAMA, T. J.; FUGANTI, R.; MARIN, S. R.; FARIAS, J. R. B.; NEUMAIER, N.; MARCELINO, F. C.; BINNECK, E.; ABDELNOOR, R. V.; NEPOMUCENO, A. L. Prospecting soybean (Glycine max L. Merrill) genes expressed under drought conditions. In: CONGRESSO BRASILEIRO DE GENÉTICA, 55., 2009, Águas de Lindóia. Charles Darwin: a origem das espécies: o livro que transformou a humanidade. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2009. p. 92.| Biblioteca(s): Embrapa Soja. |
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| 20. | | RODRIGUES, F. A.; MARCOLINO, J.; NAKAYAMA, T. J.; FUGANTI, R.; ENGELS, C.; MARIN, S. R. R.; FARIAS, J. R. B.; NEUMAIER, N.; MARCELINO, F. C.; BINNECK, E.; ABDELNOOR, R. V.; NEPOMUCENO, A. L. Prospecting soybean (Glycine Max L. Merrill) genes expressed under drought conditions. In: INTERNATIONAL CONFERENCE ON INTEGRATED APPROACHES TO IMPROVE CROP PRODUCTION UNDER DROUGHT-PRONE ENVIRONMENTS, 3., 2009, Shangai. Abstracts... Shangai: Shangai Academy of Agricultural Sciences: Shangai Agrobiological Gene Center, 2009. p. 178, ref. P 5.35. INTERDROUGHT 2009.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 22 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Soja. |
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Data corrente: |
02/10/2015 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. |
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Afiliação: |
UEL; AUTOR; AUTOR; UFV; UEL; JOSE RENATO BOUCAS FARIAS, CNPSO; U C BERKELEY - BERKELEY; HUGO BRUNO CORREA MOLINARI, CNPAE; UEL; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, v. 10, n. 9, e0139051, 2015. |
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Páginas: |
16 p. |
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DOI: |
10.1371/journal.pone.0139051 |
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Idioma: |
Inglês |
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Conteúdo: |
The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. MenosThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean ge... Mostrar Tudo |
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Palavras-Chave: |
Drought stress; Soybean. |
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Thesagro: |
Estiagem; Resistência à seca; Seca; Soja. |
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Thesaurus NAL: |
Drought tolerance; Soybeans; Transcriptome. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166647/1/nepomuceno-plos-one.pdf
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Marc: |
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024 7 $a10.1371/journal.pone.0139051$2DOI
100 1 $aMARCOLINO-GOMES, J.
245 $aTranscriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day.$h[electronic resource]
260 $c2015
300 $a16 p.
520 $aThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day.
650 $aDrought tolerance
650 $aSoybeans
650 $aTranscriptome
650 $aEstiagem
650 $aResistência à seca
650 $aSeca
650 $aSoja
653 $aDrought stress
653 $aSoybean
700 1 $aRODRIGUES, F. A.
700 1 $aFUGANTI-PAGLIARINI, R.
700 1 $aNAKAYAMA, T. J.
700 1 $aREIS, R. R.
700 1 $aFARIAS, J. R. B.
700 1 $aHARMON, F. G.
700 1 $aMOLINARI, H. B. C.
700 1 $aMOLINARI, M. D. C.
700 1 $aNEPOMUCENO, A. L.
773 $tPlos One$gv. 10, n. 9, e0139051, 2015.
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Embrapa Soja (CNPSO) |
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